Two Hybrid Services WSU Michigan Pproteome Consortium Services

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	Strains and vectors on this page are available by contacting info@twohybridservice.org Vectors The sequence files available here were assembled and annotated based on published plasmids and sequences available in Genbank. The .gb files are text files in the Genbank format. The pdf contains annotated sequences and the jpg is a map. In most cases a VectorNTI file is also available upon request. `The size of each file is in parentheses following the file name`. Bait Vectors - LexA DNA-binding domain pNLex [also known as pNLex(NLS)] HIS3 2µ ADH1p LexA NLS. Derived from pLexA(202+PL). Contains an SV40 nuclear localization signal (NLS) coding sequence downstream of LexA and upstream of the mcs. pNLex(NLS).jpg_(280kb) pNLex(NLS).gb_(20kb) pNLex(NLS).pdf_(1.2mb) pNLexAattR HIS3 2µ ADH1p LexA NLS attR. Destination version of pNLex for use with the GatewayTM (Invitrogen) in vitro cloning system. Finley et al., 2002, Gene 285, 49-57. pNLexAattR.jpg_(372kb) pNLexAattR.gb_(24kb)pNLexAattR.pdf_(1.4mb) pEG202 HIS3 2µ ADH1p LexA mcs. The most common plasmid used in the LexA two-hybrid system. Similar to pLexA(202+PL) but with a better multiple cloning site downstream of LexA. The sequence file was derived from the version marketed by Clontech and from Genbank Accession Number U89960. Estojak et al., 1995, Mol. Cell. Biol. 15, 5820-5829. pEG202.jpg_(308kb)[U89960 (GenBank site) ] pEG202.pdf_(1.2mb) pHZ5 HIS3 2µ MAL62p LexA NLS. HIS3 2µ yeast vector for regulated expression of N-terminal LexA fusions using the MAL62 promoter. Includes at the C-terminal end of LexA. This is the BD vector currently used by the Finley lab for most yeast two-hybrid studies. Finley et al., 2002, Gene 285, 49-57. pHZ5.jpg _(212kb) pHZ5.gb _(20kb) pHZ5.pdf_(1.2mb) pHZ5attR HIS3 2µ MAL62p LexA NLS attR. Destination vector version of pHZ5 for use with the GatewayTM (Invitrogen) in vitro cloning system. Finley et al., 2002, Gene 285, 49-57. pHZ5attR.jpg_(380kb) pHZ5attR.gb_(24kb)pHZ5attR.pdf_(380kb) pLexA(202+PL) HIS3 2µ ADH1p LexA. This is the granddaddy of all LexA expression vectors. HIS3 2µ with the ADH1 promoter driving expression of LexA. Most (possibly all) of the other LexA vectors shown here were derived from this common ancestor. Ruden et al., 1991, Nature 350, 250-252. pLexA(202+PL).jpg _(276kb) pLexA(202+PL).gb_(20kb) pLexA(202+PL).pdf_(1.2mb) Activation Domain Vectors pJZ4 TRP1 2µ GAL1p NLS-B42AD-HAtag mcs CYC1t. For regulated expression of AD fusions from the GAL1 promoter. Similar to pJG4-5 except with an F1 origin and the CYC1 terminator instead of ADH1t to reduce possible recombination with the BD vector. This is the AD vector currently used by the Finley lab for most yeast two-hybrid studies. pJZ4.jpg_(332kb)pJZ4.gb_(16kb) pJZ4.pdf_(952kb) pJG4-5 TRP1 2µ GAL1p NLS-B42AD-HAtag mcs ADH1t. This is the original AD vector for the Brent lab LexA two-hybrid system. The sequence file was derived from the version marketed by Clontech and from Genbank Accession Number U89961. Gyuris et al., 1993, Cell 75, 791-803. pJG4-5.jpg_(344kb) [ U89961 (GenBank site) ] pJG4-5.pdf_(920kb)