LOCUS pHZ5 9755 bp DNA circular 13-SEP-2005 SOURCE ORGANISM COMMENT The MAL61/62 promoter is in the MAL62-LexA orientation. Encodes LexA-NLS. Predicted pI = 8.73, MW = 25,525.73 Finley et al., 2002, Gene 285, 49-57 COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|194532211| COMMENT VNTDBDATE|385472808| COMMENT VNTNAME|pHZ5| COMMENT VNTAUTHORNAME|Finley Lab| COMMENT VNTAUTHORTEL|313-577-1627| COMMENT VNTAUTHORFAX|313-577-5218| COMMENT VNTAUTHORWWW|http://proteome.wayne.edu| COMMENT VNTAUTHORAD1|Center for Molecuar Medicine & Genetics| COMMENT VNTAUTHORAD2|Wayne State University School of Medicine| COMMENT VNTAUTHORAD3|540 East Canfield ave.| COMMENT VNTAUTHORAD4|Detroit, Michigan USA| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast FEATURES Location/Qualifiers CDS 906..1449 /vntifkey="4" /label=LexA\M1(TG)K2 /note="LexA coding region with two additional amino acids (TG) between M1 and K2." CDS 906..1508 /vntifkey="4" /label=LexA /note="LexA DBD (amino acids 1-202)" primer_bind 1470..1508 /vntifkey="28" /label=5'\RF126 /note="homologous to 5' end of RF126 primer" primer_bind 1491..1508 /vntifkey="28" /label=3'\3KF-BD /note="homologous to 3' end of 3KF-BD primer" primer 1141..1161 /vntifkey="27" /label=LexA\Central-s /note="LexA CDS primer, located in centre of DBD ORF" primer_bind 906..930 /vntifkey="28" /label=3'\RFx129 /note="priming site for the 3' end of RFx129. Amplifies all but ATG of LexA" primer 1482..1508 /vntifkey="27" /label=LexA\3' /note="sense primer for PCR amplification of LexA DBD plasmid inserts" primer 1484..1504 /vntifkey="27" /label=JZ45 /note="hybridize to the very end of LexA" CDS 1515..1538 /vntifkey="4" /label=NLS /note="Nuclear Localisation Sequence" primer 1243..1267 /vntifkey="27" /label=LexA-3PR /note="primer site for nested PCR of LexA-CS products and sequencing NRT junction" CDS complement(5509..6168) /vntifkey="4" /label=HIS3 /note="HIS3 gene, location according to Genbank file" CDS 7526..8386 /vntifkey="4" /label=ampR /note="ampicillin resistance gene" rep_origin <2285..>4181 /vntifkey="33" /label=2\µm\origin /note="2µm origin, the part that matches part of YEP24" terminator 1604..1934 /vntifkey="43" /label=ADH\T /note="ADH Terminator region" primer_bind complement(1659..1683) /vntifkey="28" /label=JF16 /note="anti-sense ADH T primer" primer complement(1772..1789) /vntifkey="27" /label=CAS_02 /note="anti-sense ADH T primer, flanking JF16, works well with lexA Central-s" primer_bind complement(1600..1617) /vntifkey="28" /label=pEG202R /note="reverse primer for ADH terminator region" old_sequence <8398..>9610 /vntifkey="89" /label=pBr\ori /note="pBR backbone and ori" promoter 1..896 /vntifkey="29" /label=MAL61/62\promoter /note="MAL61/62 divergent promoter region, not including each ATG or PacI site upstream of MAL61 ATG" BASE COUNT 2809 a 2124 c 2166 g 2656 t ORIGIN 1 gtcttggatg taattcttat tgttatactg aatacgctaa aaccactcac aacaagtatg 61 gagtatattg tgtctcttta tatgctgagt acttatgcaa tatgcgctca ctcaggatga 121 aatgtacaca gccgaaagta tattgaaagc tgcctctgtg gaaacttcta tctaatcttg 181 tctccagatg tagactatga ggcctgaaga agtctttagg cacctgttgg agagtataag 241 gagactgcta caacaacgtc ttcccacaaa aaattatctg gaggccggta tgatacctgc 301 acaaaggtta agttacacat gaaaaagaaa ctgacataac tttgatctct gaaaatatct 361 tttcccctga gtagcttcac tgcttggata ccaataggaa tagaccttgg ctatagtaag 421 ttgcatctgt accgtagaga ttcttgcaac tcgcttaaac tctcgctttt agataatatt 481 tctccttatt gcgcgcttcg ttgaaaattt cgctaaacac ggggtttaag ttaaagttta 541 caggatttat ccggaaattt tcgcggaccc cacacaatta agaattggct cgaagagtga 601 taacgcatac ttttcttttc tttttttagt tcctagcgta cctaacgtag gtaacatgat 661 ttggatcgtg ggatgataca aacaacgtaa gatgactact tccttcctca attcttcttg 721 cagcatcatt ttcttgaggc gctctgggca aggtataaaa agttccatta atacgtctct 781 aaaaaattaa atcatccatc tcttaagcag tttttttgat aatctcaaat gtacatcagt 841 caagcgtaac taaattacat aaatgtacat cagtcaagcg taactaaatt acataaatga 901 ccggtaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt gatcacatca 961 gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc 1021 caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt 1081 ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg ccgctggtag 1141 gtcgtgtggc tgccggtgaa ccacttctgg cgcaacagca tattgaaggt cattatcagg 1201 tcgatccttc cttattcaag ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga 1261 tgaaagatat cggcattatg gatggtgact tgctggcagt gcataaaact caggatgtac 1321 gtaacggtca ggtcgttgtc gcacgtattg atgacgaagt taccgttaag cgcctgaaaa 1381 aacagggcaa taaagtcgaa ctgttgccag aaaatagcga gtttaaacca attgtcgtag 1441 atcttcgtca gcagagcttc accattgaag ggctggcggt tggggttatt cgcaacggcg 1501 actggctgga attggccccc aagaaaaaga gaaaggtgga attcccgggg atccgtcgac 1561 catggcggcc gctcgagtcg acctgcagcc aagctaattc cgggcgaatt tcttatgatt 1621 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga 1681 ctcttaggtt ttaaaacgaa aattcttgtt cttgagtaac tctttcctgt aggtcaggtt 1741 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag 1801 caaatgcctg caaatcgctc cccatttcac ccaattgtag atatgctaac tccagcaatg 1861 agttgatgaa tctcggtgtg tattttatgt cctcagagga caacacctgt tgtaatcgtt 1921 cttccacacg gatcgatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg 1981 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg 2041 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga 2101 ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc 2161 aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta 2221 gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa 2281 agctagcttt gaagaaaaat gcgccttatt caatctttgc tataaaaaat ggcccaaaat 2341 ctcacattgg aagacatttg atgacctcat ttctttcaat gaagggccta acggagttga 2401 ctaatgttgt gggaaattgg agcgataagc gtgcttctgc cgtggccagg acaacgtata 2461 ctcatcagat aacagcaata cctgatcact acttcgcact agtttctcgg tactatgcat 2521 atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt 2581 ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga 2641 atgggataat atcacaggag gtactagact acctttcatc ctacataaat agacgcatat 2701 aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca 2761 ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg 2821 cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt 2881 ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca 2941 ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg 3001 cttccacaaa cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat 3061 ataacctacc catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt 3121 tttatgttta tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata 3181 gagtgaatcg aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa 3241 aatagaagaa accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc 3301 tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct 3361 tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct 3421 ttttttatgg aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac 3481 agtgcaaaaa gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc 3541 taagatgctt tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa 3601 gtatagattc tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg 3661 cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa 3721 atgaagcaca gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa 3781 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta 3841 caaaatgaag cacagatgct tcgttaacaa agatatgcta ttgaagtgca agatggaaac 3901 gcagaaaatg aaccggggat gcgacgtgca agattaccta tgcaatagat gcaatagttt 3961 ctccaggaac cgaaatacat acattgtctt ccgtaaagcg ctagactata tattattata 4021 caggttcaaa tatactatct gtttcaggga aaactcccag gttcggatgt tcaaaattca 4081 atgatgggta acaagtacga tcgtaaatct gtaaaacagt ttgtcggata ttaggctgta 4141 tctcctcaaa gcgtattcga atatcattga gaagctgcag caggcgtgaa gttagacgac 4201 aacttctctc tggaaacgca taccgatatt caggctgctg caaaggcaca ggctagtgcc 4261 cgtgcgagtg catccggtac caccccagat gctgtagtag cttctggtag cactgcaatg 4321 agccatgctt atcaagaaaa cacaggtttt ggtactcgtc ccatatatct tgacatgcaa 4381 gccactacac caacagaccc tagggttttg gatacgatgt tgaagtttta tacgggactt 4441 tatggtaatc ctcattccaa cactcactct tacggttggg aaacaaatac tgctgtggaa 4501 aatgctagag ctcacgtagc aaagatgatc aatgccgacc ccaaggaaat aatattcact 4561 tcgggagcga ccgaatctaa taatatggtt cttaagggtg tcccaagatt ttataagaag 4621 actaagaaac acatcatcac cactagaacg gaacacaagt gtgtcttgga agccgcacgg 4681 gccatgatga aggagggatt tgaagtcact ttcctaaatg tggacgatca aggtcttatc 4741 gatttgaagg aattggaaga tgccattaga ccagatacct gtctcgtctc tgtgatggct 4801 gtcaataatg aaatcggtgt cattcaacct attaaagaaa ttggagcaat ttgtagaaag 4861 aataagatcc tcggggacac caaatatggc gatctcggcc ttttcgtttc ttggagctgg 4921 gacatgtttg ccatcgatcc atctaccacc agaacggccg ttagatctgc tgccaccgtt 4981 gtttccaccg aagaaaccac cgttgccgta accaccacga cggttgttgc taaagaagct 5041 gccaccgcca cggccaccgt tgtagccgcc gttgttgtta ttgtagttgc tactgttatt 5101 tctggcactt cttggttttc ctcttaagtg aggaggaaca taaccattct cgttgttgtc 5161 gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa atgcttttct 5221 tgttgttctt acggaatacc acttgccacc tatcaccaca actaactttt tcccgttcct 5281 ccatctcttt tatatttttt ttctcgatcg agttcaagag aaaaaaaaag aaaaagcaaa 5341 aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg cattaccttg 5401 tcatcttcag tatcatactg ttcgtataca tacttactga cattcatagg tatacatata 5461 tacacatgta tatatatcgt atgctgcagc tttaaataat cggtgtcact acataagaac 5521 acctttggtg gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac 5581 cgcaagagcc ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc 5641 aacgtggagg gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc 5701 gcaagagaga tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct 5761 gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc 5821 aaaccttttt actccacgcg ccagtagggc ctctttaaaa gcttgaccga gagcaatccc 5881 gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact caacgattag 5941 cgaccagccg gaatgcttgg ccagagcatg tatcatatgg tccagaaacc ctatacctgt 6001 gtggacgtta atcacttgcg attgtgtggc ctgttctgct actgcttctg cctctttttc 6061 tgggaagatc gagtgctcta tcgctagggg accacccttt aaagagatcg caatctgaat 6121 cttggtttca tttgtaatac gctttactag ggctttctgc tctgtcatct ttgccttcgt 6181 ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa atcacattac tttatataat 6241 gtataattca ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca tccgctaggt 6301 ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg tactagagga 6361 ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac ttccctgact 6421 aatgccgtgt tcaaacgata cctggcagtg actcctagcg ctcaccaagc tcttaaaacg 6481 agaattaaga aaaagtcgtc atctttcgat aagtttttcc cacagcaaag caatagtaga 6541 aaaacaatgg gaaacgttga atgaagacaa agcgtcgtgg tttaaaagga aatacgctca 6601 cgtacatgct agggaacagg accgtgcagc ggatctaatg aatccatttg ttagttaata 6661 gtttaaatgt ttttatcgga agaggttttg tcatcacatc agcaatgttc ttcttggtct 6721 cgatgtagta tacgtataaa ttattacctg atacttcatc tctaagtctc attgcctttg 6781 tgccaaaaaa tctgtttcta aatttctctt catttgtaga cttaattata ctgatcgttg 6841 atctactatc agtaagtaag cctttaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaacctg 6901 taacaatagc aataccccaa atacctaatg tagttccagc aagcaagcta aaaagtaaag 6961 caacaacata actcacccct gcatctgcag cttttgcccg ggcagcctgc tctgcctgtg 7021 ttttctttaa ttgagcagta gaccatttag cagttgcatg aatagctgca gcgtcacatc 7081 ggataataat gatggcagcc attgtagaag tgccttttgc atttctagtc tctttctcgg 7141 tctagctagt tttactacat cgcgaagata gaatcttaga tcacactgcc tttgctgagc 7201 tggatcaata gagtaacaaa agagtggtaa ggcctcgtta aaggacaagg acctgagcgg 7261 aagtgtatcg tacagtagac ggagtatact agtatagtct atagtccgtg gaattaattc 7321 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 7381 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 7441 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 7501 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 7561 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 7621 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 7681 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 7741 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg 7801 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 7861 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 7921 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 7981 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 8041 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt 8101 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 8161 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 8221 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 8281 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 8341 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 8401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 8461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 8521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 8581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 8641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 8701 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 8761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 8821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 8881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 8941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 9001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 9061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 9121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 9181 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 9241 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 9301 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct 9361 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata 9421 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac 9481 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 9541 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 9601 cgcgcgaggc aggatgatcc gggatcgaag aaatgatggt aaatgaaata ggaaatcaag 9661 gagcatgaag gcaaaagaca aatataaggg tcgaacgaaa aataaagtga aaagtgttga 9721 tatgatgtat ttggctttgc ggcgattaat taata //